EFFECT OF GLYCOSAMINOGLYCANS ON OPENING ANGLE OF INTACT & CROSSLINKED AORTAS

BackgroundRuptures caused by aortic aneurysms and dissections occur when the mechanical stresses in wall exceed local strength. In-vivo are strongly mediated residual (RS), those existing absence of load. Circumferential RS can be estimated ex-vivo via opening angle (OA), subjecting an ring to a radial cut. Although underlying mechanism for remains poorly understood, our recent efforts have demonstrated strong correlations between amounts sulfated glycosaminoglycans (sGAG) OA healthy aortas. In addition, advanced glycation end products (AGEs) known accumulate with age create crosslinks within extracellular matrix. Since ruptures more prevalent older tissue, purpose this study was compare effect sGAG on intact glycated tissue.Methods ResultsSets four adjacent rings were excised from upper thoracic regions 9 porcine One served as control, while second underwent enzymatic depletion, third glycation, fourth combination followed depletion. A 100mM ammonium acetate buffer, pH 7.0 used. Glycation depletion induced incubating samples 700mM ribose, 15U/mL hyaluronidase, 0.075U/mL chondroitinase ABC, 0.75U/mL heparinase 48 hours at 37oC, respectively. The then measured general AGE contents quantified. quantification control treated confirmed successful removal sGAG, reductions 94±2.5% (average ± standard deviation). 93±27% increase AGEs achieved. Statistically significant differences found OAs depleted, glycated, combined depleted (paired sample t-test, p< 0.001). Specifically, reduced 34±12% after 14±8% 45±15% samples, well significantly smaller than that only p=0.002), being 22±15% 34±18% respectively.ConclusionThese findings supported causes reduction OA, both may also affect magnitude RS. tissue. Ruptures Methods Sets ConclusionThese These